Aptagen, LLC
DNA
HDLM2
Cells
10 nM (reported value)
Washing buffer for selection: Dulbeccoƒ??s PBS with 4.5 g/L glucose and 5 mM MgCl2s. Binding buffer to reduce nonspecific background binding: 1 mg/mL BSA and 0.1 mg/mL t-RNA
°C
Heat at 95°C for 5 mins
ssDNA library for SELEX contains a 18 mer primer binding region at the 5' and 3' end with a 30 mer randomized region in the middle.
Hodgkin Lymphoma aptamer (ID# 525)
198
20312.25 g/mole
631700 L/(mole·cm)
46.97%
1.58
32.15
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Parekh, P. et al. "Biostable ssDNA Aptamers Specific for Hodgkin Lymphoma." Sensors 2013, 13: 14543-14557.
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