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Apta-Index™: Hodgkin Lymphoma aptamer (ID# 525)

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Hodgkin Lymphoma aptamer (ID# 525)

Hodgkin Lymphoma aptamer
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA HDLM2 Cells 10 nM (reported value) Washing buffer for selection: Dulbecco’s PBS with 4.5 g/L glucose and 5 mM MgCl2s. Binding buffer to reduce nonspecific background binding: 1 mg/mL BSA and 0.1 mg/mL t-RNA Heat at 95°C for 5 mins ssDNA library for SELEX contains a 18 mer primer binding region at the 5' and 3' end with a 30 mer randomized region in the middle.
dTpdApdCpdCpdApdGpdTpdGpdCpdGpdApdTpdGpdCpdTpdCpdApdGpdTpdApdApdCpdTpdTpdTpdGpdApdApdGpdGpdApdApdApdGpdGpdCpdTpdApdCpdApdApdApdCpdTpdCpdTpdTpdCpdCpdTpdGpdApdCpdGpdCpdApdTpdTpdCpdGpdGpdTpdTpdGpdApdCp
66 20312.25 g/mole 631700.00 L/(mole·cm) 46.97% 1.58 32.15

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Parekh, P. et al. "Biostable ssDNA Aptamers Specific for Hodgkin Lymphoma." Sensors 2013, 13: 14543-14557.

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