Aptagen, LLC
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
2'-O-Me-RNA
p53
Protein
N/A nM (reported value)
0.15 mM Hepes [pH 7.4], 0.3 mM KCl, 6 uM dithiothreitol [DTT], 6 uM MgCl2, 1.1 uM ethylenediaminetetraacetic acid [EDTA], 6% glycerol, and 3 uM phenylmethanesulfonyl fluoride [PMSF]
23°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
2'-O-Methyl modified RNA aptamer (ID# 512)
5'mGpmGpmGpmCpmGpmApmApmUpmUpmCpmGpmGpmGpmUpmUpmGpmGpmApmUpmApmGpmUpmApmGpmGpmCpmGpmCpmApmUpmApmUpmGpmGpmCpmApmUpmCpmUpmUpmCpmGpmUpmGpmGpmUpmUpmGpmUpmGpmUpmApmUpmUpmGpmCpmCpmCpmUpmUpmUpmApmGpmUpmGpmApmGpmGpmGpmUpmUpmApmApmUpmUp3'
75
25257.7 g/mole
753400 L/(mole·cm)
48.00%
1.33
33.52
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Shwetha, N. et al. "Aptamerƒ??nanoparticle-based chemiluminescence for p53 protein." Analytical Biochemistry 2013, 441: 73-79.
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