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Apta-Index™: TTF1 (A) (ID# 51)

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TTF1 (A) (ID# 51)

TTF1 (A)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Thyroid Transcription Factor 1 Protein 3.36 nM (reported value) Ni-NTA magnetic bead (Qiagen) were used with PBS-T (50 mM K2HPO4 pH 7.5, 150 mM NaCl, 0.05% Tween-20) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-dGpdGpdTpdApdTpdTpdGpdApdGpdGpdGpdTpdCpdGpdCpdApdTpdCpdTpdCpdApdApdApdApdGpdGpdGpdGpdTpdGpdApdTpdTpdGpdCpdTpdTpdGpdCpdApdCpdApdApdTpdGpdApdCpdApdGpdGpdGpdTpdApdGpdGpdApdCpdApdGpdApdTpdGpdGpdCpdTpdCpdTpdApdApdCpdTpdCpdTpdCpdCpdTpdCpdTp-3'
78 24171.74 g/mole 753300.00 L/(mole·cm) 50.00% 1.33 32.09

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Murphy et al. "An improved method for the in vitro evolution of aptamers and applications in protein detection and purification." Nucleic Acids Research, 31 (2003): e110.

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