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Apta-Index™: Anti Anterior Gradient Homolog 2 (ID# 508)

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Anti Anterior Gradient Homolog 2 (ID# 508)

Anti Anterior Gradient Homolog 2
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Anterior Gradient Homolog 2 (AGR2) Protein 13.16 nM (reported value) 200 mL, 1X PBS buffer containing 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4 and 2.0 mM KH2PO4, pH 7.4 heat at 95C for 5 minutes, then, cool on ice for 10 minutes and finally incubate at room temperature for 10 minutes : The Mfold structure shown here is the secondary structure of the original aptamer and its primers at selection conditions. However, in the reference paper the authors do not give this secondary structure but a structure consisting of the sequenced random region with a Streptavidin aptamer. That, is a design for a molecular beacon not necessarily the aptamer sequence.
5'-dTpdCpdTpdCpdGpdGpdApdCpdGpdCpdGpdTpdGpdTpdGpdGpdTpdCpdGpdGpdGpdTpdGpdGpdGpdApdGpdTpdTpdGpdTpdGpdGpdGpdGpdGpdGpdGpdGpdGpdTpdGpdGpdGpdApdGpdGpdGpdTpdTpdCpdTpdTpdTpdGpdTpdTpdTpdGpdApdTpdCpdTpdTpdTpdCpdTpdCpdGpdCpdTpdGpdCpdCpdTpdGpdGpdCpdCpdCpdTpdApdGpdApdGpdTpdGp-3'
87 27207.59 g/mole 804600.00 L/(mole·cm) 62.07% 1.24 33.82

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Wu et al. Identification, Characterization and Application of a G-Quadruplex Structured DNA Aptamer against Cancer Biomarker Protein Anterior Gradient Homolog 2 (PLOSone)

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