Aptamer Details

Anti Lung Carcinoma ( EJ4) (ID# 507)

Aptamer Chemistry: DNA Target: Lung Adenocarcinoma (H23) Antigen/Target Category: Cells Affinity (Kd): 60 nM (reported value) Binding Conditions/Buffer: 0.45% w/v glucose 5 mM MgCl2 in PBS 1 mg/mL tRNA 1 mg/mL BSA incubate with cells for 30 minutes Binding Temp: 4°C Refolding Protocol: denature by heating at 95C for 5 minutes, followed by rapid cooling on ice Specificity: HBE 135-E6/E7 (normal epithelial lung) cells used as counter targets Comments: Reference did not list what primer sequences they used. The Author only mentioned that the primers were designed using IDTƒ??s oligo analyzer and met the following characteristics: a minimum hairpin structure, similar melting temperatures and minimal base pairing.

Sequence (): 5'dGpdApdApdGpdApdCpdGpdApdGpdCpdGpdGpdCpdGpdApdGpdTpdGpdTpdTpdApdTpdTpdApdCpdGpdCpdTpdTpdGpdGpdApdApdApdCpdApdApdCpdCpdCpdCp3' Length: 41 Molecular Weight: 12668.28 g/mole Extinction Coefficient: 402800 L/(mole·cm) GC Content: 53.66% nmoles/OD₂₆₀: 2.48 μg/OD₂₆₀: 31.45

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Jimenez et al. Generation of Lung Adenocarcinoma DNA Aptamers for Cancer Studies (PLOSone)

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Aptamer Details

Anti Lung Carcinoma ( EJ4) (ID# 507)

Jimenez et al. Generation of Lung Adenocarcinoma DNA Aptamers for Cancer Studies (PLOSone)

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