Aptamer Details

Leptin (Lep3) (ID# 500)

Leptin (Lep3)
DNA Leptin Protein 0.32 µM (reported value) TGK 1X: 25 mM Tris, 192 mM glycine, and 5 mM potassium phosphate (pH 8.3) 20°C Heated to 94°C and cooled at a rate of 0.5°C/s to 20°C. Showed ~10 fold discrimination between β-lactoglobulin (Kd=2.3 μM) and bovine catalase (Kd=3.1 μM). This aptamer was selected using nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM). Binding affinity against immobilized leptin was much lower (1.50 μM).
5' dCpdTpdTpdCpdTpdGpdCpdCpdCpdGp dCpdCpdTpdCpdCpdTpdTpdCpdCpdGp dTpdTpdApdApdTpdGpdGpdGpdGpdGp dApdTpdCpdTpdCpdGpdCpdGpdGpdCp dCpdGpdTpdTpdCpdTpdTpdGpdTpdTp dGpdCpdTpdTpdApdTpdApdCpdApdGp dGpdApdGpdApdCpdGpdApdGpdApdTp dApdGpdGpdCpdGpdGpdApdCpdApdCp dTp3' 84 24925.14 g/mole 744800 L/(mole·cm) 56.79% 1.34 33.47

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Ashley J and Li S FY. (2013) Three-dimensional selection of leptin aptamers using capillary electrophoresis and implications for clone validation. Anal Biochem. 434: 146-152.

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