Aptagen, LLC
DNA
Leptin
Protein
0.32 µM (reported value)
TGK 1X: 25 mM Tris, 192 mM glycine, and 5 mM potassium phosphate (pH 8.3)
20°C
Heated to 94°C and cooled at a rate of 0.5°C/s to 20°C.
Showed ~10 fold discrimination between β-lactoglobulin (Kd=2.3 μM) and bovine catalase (Kd=3.1 μM).
This aptamer was selected using nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM). Binding affinity against immobilized leptin was much lower (1.50 μM).
Leptin (Lep3) (ID# 500)
5' dCpdTpdTpdCpdTpdGpdCpdCpdCpdGp dCpdCpdTpdCpdCpdTpdTpdCpdCpdGp dTpdTpdApdApdTpdGpdGpdGpdGpdGp dApdTpdCpdTpdCpdGpdCpdGpdGpdCp dCpdGpdTpdTpdCpdTpdTpdGpdTpdTp dGpdCpdTpdTpdApdTpdApdCpdApdGp dGpdApdGpdApdCpdGpdApdGpdApdTp dApdGpdGpdCpdGpdGpdApdCpdApdCp dTp3'
84
24925.14 g/mole
744800 L/(mole·cm)
56.79%
1.34
33.47
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Ashley J and Li S FY. (2013) Three-dimensional selection of leptin aptamers using capillary electrophoresis and implications for clone validation. Anal Biochem. 434: 146-152.
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