* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Leptin Protein 0.32 µM (reported value)TGK 1X: 25 mM Tris, 192 mM glycine, and 5 mM potassium phosphate (pH 8.3)Heated to 94°C and cooled at a rate of 0.5°C/s to 20°C.
This aptamer was selected using nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM). Binding affinity against immobilized leptin was much lower (1.50 μM).
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Ashley J and Li S FY. (2013) Three-dimensional selection of leptin aptamers using capillary electrophoresis and implications for clone validation. Anal Biochem.434: 146-152.
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* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA
Leptin
Protein
0.32 µM (reported value)
TGK 1X: 25 mM Tris, 192 mM glycine, and 5 mM potassium phosphate (pH 8.3)
Heated to 94°C and cooled at a rate of 0.5°C/s to 20°C.
This aptamer was selected using nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM). Binding affinity against immobilized leptin was much lower (1.50 μM).
