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Apta-Index™: Kanamycin A (#13_82) (ID# 498)

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Kanamycin A (#13_82) (ID# 498)

Kanamycin A (#13_82)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Kanamycin A Small Organic 5.1 µM (reported value) Selection Buffer: 100 mM NaCL, 20 mM Tris-HCl pH 7.6, 2 mM MgCl2, 5 mM KCl, and 1 mM CaCl2 90°C for 8 min, immediately cooled, 4 °C for 10 min. Lowercase letters denote constant regions. This aptamer was selected using capture-SELEX in which the DNA was immoblized using DNA complimentary to the middle constant region and was eluted by the target during the selection process.
5'-dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdCpdApdGpdGpdGpdCpdGpdGpdTpdApdTpdGpdApdGpdGpdCpdTpdCpdGpdApdTpdCpdApdApdGpdGpdTpdCpdGpdGpdApdGpdCpdGpdApdGpdApdApdTpdTpdTpdTpdTpdTpdCpdGpdCpdGpdGpdApdGpdTpdCpdGpdGpdCpdTpdGpdGpdApdTpdCpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp-3'
98 30429.82 g/mole 961600.00 L/(mole·cm) 47.96% 1.04 31.64

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Stoltenburg R, Nikolaus N, Strehlitz B.(2012) Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics. J Anal Methods Chem. 2012:doi:10.1155/2012/415697

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