Aptamer Details

Terminal 8-oxodG DNA Lesion (R10-B35) (ID# 488)

Aptamer Chemistry: RNA Target: DNA Lesion 7,8-dihydro-8-hydroxy-2'-deoxyguanosine (8-oxodG) Antigen/Target Category: Small Organic Affinity (Kd): 5 µM (reported value) Binding Conditions/Buffer: Binding buffer: 20 mM NH₄CH₃CO₂ (pH 6.0), 300 mM NaCl, and 5 mM MgCl₂. Binding Temp: RT°C Refolding Protocol: Denatured in boiling water bath for 1 minute and then mixed with 10 ul of 200 mM NH₄CH₃CO₂ (pH 6.0), 3 M NaCl, and 50 mM MgCl2, and allowed to renature for 1 hour. Comments:

Sequence (): 5'rGprGprGprCprGprAprAprUprUprCprCprCprGprAprGprGprAprCprCprAprAprAprUprAprGprUprAprCprCprAprCprCprCprGprGprGprAprAprAprAprCprAprGprCprUprAprAprUprGprCprCprGprAprAprAprCprGprGprAprGprAprUprUprUprUprUprUprCprUprGprCprAprGprAprAprGprCprUp3' Length: 78 Molecular Weight: 25157.29 g/mole Extinction Coefficient: 782200 L/(mole·cm) GC Content: 50.00% nmoles/OD₂₆₀: 1.28 μg/OD₂₆₀: 32.16

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Rink, S. M. et al. "Creation of RNA molecules that recognize the oxidative lesion 7,8-dihydro-8-hydroxy-2-deoxyguanosine (8-oxodG) in DNA." Proc. Natl. Acad. Sci.*, 95 (1998):11619-11624.

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Aptamer Details

Terminal 8-oxodG DNA Lesion (R10-B35) (ID# 488)

Rink, S. M. et al. "Creation of RNA molecules that recognize the oxidative lesion 7,8-dihydro-8-hydroxy-2*-deoxyguanosine (8-oxodG) in DNA." Proc. Natl. Acad. Sci., 95 (1998):11619-11624.

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Rink, S. M. et al. "Creation of RNA molecules that recognize the oxidative lesion 7,8-dihydro-8-hydroxy-2-deoxyguanosine (8-oxodG) in DNA." Proc. Natl. Acad. Sci.*, 95 (1998):11619-11624.