Aptamer Details

Bacillus anthracis spores (BAS-6F) (ID# 487)

Aptamer Chemistry: DNA Target: Bacillus anthracis spores Antigen/Target Category: Other Affinity (Kd): N/A nM (reported value) Binding Conditions/Buffer: Binding buffer: 0.5 M NaCl, 10 mM Tris-HCl, 1 mM MgCl₂ (pH 7.5-7.6) Binding Temp: RT°C Refolding Protocol: Heated to 95° C for 5 minutes and added hot. Specificity: binds to bacillus antracis over anthrose sugar. Comments:

Sequence (): 5'dApdTpdApdCpdGpdGpdGpdApdGpdCpdCpdApdApdCpdApdCpdCpdApdTpdCpdCpdCpdTpdCpdTpdTpdApdGpdGpdApdTpdApdCpdApdApdApdGpdCpdCpdApdApdApdCpdTpdGpdApdGpdCpdCpdCpdGpdTpdGpdCpdApdGpdApdGpdCpdApdGpdGpdTpdGpdTpdGpdApdCpdGpdGpdApdTp3' Length: 72 Molecular Weight: 22213.45 g/mole Extinction Coefficient: 706300 L/(mole·cm) GC Content: 54.17% nmoles/OD₂₆₀: 1.42 μg/OD₂₆₀: 31.45

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Bruno, J. G. and Carrillo, M. P. "Development of aptamer beacons for rapid presumptive detection of Bacillus spores." J Fluoresc, 22 (2012): 915-924.

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Aptamer Details

Bacillus anthracis spores (BAS-6F) (ID# 487)

Bruno, J. G. and Carrillo, M. P. "Development of aptamer beacons for rapid presumptive detection of Bacillus spores." J Fluoresc, 22 (2012): 915-924.

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