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Bacillus anthracis spores (Ba) (ID# 486)

Aptamer Chemistry: DNA Target: Bacillus anthracis spores Antigen/Target Category: Other Affinity (Kd): 1.5x10^12 CFU/mL (reported value) Binding Conditions/Buffer: Reaction buffer: 350 uL 2 M Tris-HCl, 100 uL 1 M MgCl₂, 7.7 mg dithiothreitol, and water Binding Temp: 37°C Refolding Protocol: Stir win MOPS buffer at 60° C for one day. Then, slowly cool to room temperature. Comments:

Sequence (): 5'dApdCpdCpdCpdCpdTpdGpdCpdApdTpdCpdCpdTpdTpdTpdGpdCpdTpdGpdGpdApdGpdApdGpdGpdApdApdTpdGpdTpdApdTpdApdApdGpdGpdApdTpdGpdTpdTpdCpdCpdGpdGpdGpdCpdGpdTpdGpdTpdGpdGpdGpdTpdApdApdGpdTpdCpdApdGpdTpdCpdTpdApdGpdApdGpdGpdGpdCpdCpdCpdCpdApdGpdApdApdTp3' Length: 80 Molecular Weight: 24831.14 g/mole Extinction Coefficient: 776600 L/(mole·cm) GC Content: 53.75% nmoles/OD₂₆₀: 1.29 μg/OD₂₆₀: 31.97

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Fan, M. et al. "Aptamer selection express: A novel method for rapid single-step selection and sensing of aptamers." Journal of Biomolecular Technologies, 19 (2008): 311-321.

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Aptamer Details

Bacillus anthracis spores (Ba) (ID# 486)

Fan, M. et al. "Aptamer selection express: A novel method for rapid single-step selection and sensing of aptamers." Journal of Biomolecular Technologies, 19 (2008): 311-321.

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