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Apta-Index™: von Willebrand Factor (VWF R9.14) (ID# 482)

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von Willebrand Factor (VWF R9.14) (ID# 482)

von Willebrand Factor (VWF R9.14)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
2'-F-RNA von Willebrand Factor (VWF) Protein 12 nM (reported value) For nitrocellulose filter binding assays:
Selection Buffer E (20mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA)
-OR-
Selection Buffer F (20mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, and 0.1% BSA)

For platelet function analysis(performed at RT):
Platelet binding buffer: 150 mM NaCl, 20 mM Hepes pH 7.5; 5 mM KCL; 1 mM MgCl2, and 1 M CaCl2.
N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Binds to the A1 domain of vWF and completely inhibits ristocetin-induced platelet aggregation at a concentration of 250 nM.

Inhibition was reversed using complementary sequence:
GGTUCCTCAGCGAGUCGUCUG
5'-rGprGprGprAprGprGprApfCprGprApfUprGpfCprGprGp fUprGprGp rApfCprGp rAprApfCp fUprGpfCp fCpfCpfUp fCprAprGp fCpfUprAp fCpfUpfUp fUpfCprAp fUprGpfUp fUprGpfCp fUprGprAp fCprGpfCp rAp fCprAprGprApfCprGprApfCpfUpfCprGpfCpfUprGprAprGprGprApfUpfCpfCp -3'
76 24563.62 g/mole 731400.00 L/(mole·cm) 59.21% 1.37 33.58

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

S Oney, BA Sullenger, et al. (2007) "Antidote-Controlled Platelet Inhibition Targeting von Willebrand Factor with Aptamers." Oligonucleotides, 17:265-274.

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