Aptagen, LLC
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
2'-F-RNA
von Willebrand Factor (VWF)
Protein
12 nM (reported value)
For nitrocellulose filter binding assays:
Selection Buffer E (20mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA)
-OR-
Selection Buffer F (20mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, and 0.1% BSA)
For platelet function analysis(performed at RT):
Platelet binding buffer: 150 mM NaCl, 20 mM Hepes pH 7.5; 5 mM KCL; 1 mM MgCl2, and 1 M CaCl2. 37°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Specifically inhibited ristocetin-induced platelet aggregation and not collagen-induced, ADP-induced, or thrombin-induced platelet aggregation. Binds to the A1 domain of vWF and completely inhibits ristocetin-induced platelet aggregation at a concentration of 250 nM.
Inhibition was reversed using complementary sequence:
GGTUCCTCAGCGAGUCGUCUG
von Willebrand Factor (VWF R9.14) (ID# 482)
Selection Buffer E (20mM HEPES (pH 7.4), 50 mM NaCl, 2 mM CaCl2, and 0.1% BSA)
-OR-
Selection Buffer F (20mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, and 0.1% BSA)
For platelet function analysis(performed at RT):
Platelet binding buffer: 150 mM NaCl, 20 mM Hepes pH 7.5; 5 mM KCL; 1 mM MgCl2, and 1 M CaCl2. 37°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Specifically inhibited ristocetin-induced platelet aggregation and not collagen-induced, ADP-induced, or thrombin-induced platelet aggregation. Binds to the A1 domain of vWF and completely inhibits ristocetin-induced platelet aggregation at a concentration of 250 nM.
Inhibition was reversed using complementary sequence:
GGTUCCTCAGCGAGUCGUCUG
5'rGprGprGprAprGprGprApfCprGprApfUprGpfCprGprGp fUprGprGp rApfCprGp rAprApfCp fUprGpfCp fCpfCpfUp fCprAprGp fCpfUprAp fCpfUpfUp fUpfCprAp fUprGpfUp fUprGpfCp fUprGprAp fCprGpfCp rAp fCprAprGprApfCprGprApfCpfUpfCprGpfCpfUprGprAprGprGprApfUpfCpfCp 3'
82
24563.62 g/mole
731400 L/(mole·cm)
59.21%
1.37
33.58
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
S Oney, BA Sullenger, et al. (2007) "Antidote-Controlled Platelet Inhibition Targeting von Willebrand Factor with Aptamers." Oligonucleotides, 17:265-274.
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