Aptagen, LLC
DNA
Lysergamine, metergoline, and other small ergot alkaloids
Protein
44 nM (reported value)
Binding Buffer: 100 mM NaCl, 20 mM Tris-HCl (pH 7.6), 5 mM KCl, 2 mM MgCl2 ?ú6 H2O, 1 mM CaCl2?ú2 H2O.
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Only the Kd value for lysergamine was reported.
The colorimetric assay responded to lysergamine, metergoline, and an ergot-containing flour extract. The aptamer contains two cytosine-repeat binding sites.
Lysergamine (M3.2) (ID# 470)
The colorimetric assay responded to lysergamine, metergoline, and an ergot-containing flour extract. The aptamer contains two cytosine-repeat binding sites.
5'dApdGpdCp dApdGpdCp dApdCpdAp dGpdApdGp dGpdTpdCp dApdGpdAp dTpdGpdGp dCpdGpdTp dCpdApdGp dCpdCpdCp dCpdGpdAp dTpdCpdGp dCpdCpdAp dTpdCpdCp dApdGpdGp dGpdApdCp dTpdCpdCp dCpdCpdCp dCpdTpdAp dCpdTpdGp dCpdCp dTpdApdTp dGpdCpdGp dTpdGpdCp dTpdApdCp dCpdGpdTp dGpdApdAp3'
88
24516.84 g/mole
747200 L/(mole·cm)
62.50%
1.34
32.81
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
ER Martin et al. (2012) Aptamer-based molecular recognition of lysergamine, metergoline and small ergot alkaloids. Int. J. Mol. Sci. 13: 17138-17159.
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