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Apta-Index™: Peroxidase DNAzyme ([B7]-3-0) (ID# 469)

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Peroxidase DNAzyme ([B7]-3-0) (ID# 469)

Peroxidase DNAzyme ([B7]-3-0)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Hemin Protein 29 nM (reported value) Binding buffer: 20 mM Tris-AcOH (pH 8.0), 100 mM NaCl, 200 mM KCl, 5 mM MgCl2, 0.5% Triton X-100, and 5% DMSO.

Peroxidase activity was measured using the ABTS-H2O2 system.
Heated at 95°C for 5 min in 40KT buffer, cooled on ice for 5 min, and placed at RT.

40KT buffer: 50 mM MES (pH 6.5), 100 mM Tris acetate, 40 mM potassium acetate, 1% DMSO, and 0.05% Triton X
Forms a G-quadruplex structure that enhances the natural peroxidase activity of hemin
20 6349.19 g/mole 203600.00 L/(mole·cm) 60.00% 4.91 31.18

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Zhu L, et al. (2012) In vitro selection of highly efficient G-quadruplex-based DNAzymes. Anal. Chem. 84: 8383-8390.

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