Aptagen, LLC
RNA
Kanamycin A
Protein
<300 nM (reported value)
Binding Buffer (500 mM NaCl, 50 mM Tris Cl (pH 7.6))
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Moderate binding to dibekacin, amikacin, and ribostamycin observed. No binding to bekanamycin or streptomycin observed.
Kanamycin A (sla 110) (ID# 466)
5'rGprGprGp rAprAprUp rGprGprAp rUprCprCp rAprCprAp rUprCprUp rAprCprGp rAprAprUp rUprCprGp rAprAprUp rUprGprCp rCprGprUp rAprAprUp rUprUprCp rCprCprGp rUprGprGp rAprGprCp rGprAprUp rGprCprUp rUprCprAp rCprUprGp rCprAprGp rAprCprUp rUprGprAp rCprGprAp rAprGprCp rUprUp3'
88
25654.43 g/mole
787300 L/(mole·cm)
48.75%
1.27
32.59
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Lato et al. "In vitro selection of RNA lectins: using combinatorial chemistry to interpret ribozyme evolution." Chemistry & Biology, 2(1995): 291-303.
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