Aptagen, LLC
DNA
Hen egg white lysozyme and recombinant human lysozyme
Protein
3-Feb nM (reported value)
Binding Buffer: 25 mM trizma base, 192 mM glycine, and 5 mM K2HPO4, pH 8.3.
25°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Tran et al. found good specificity against BSA, ovomucoid, and ovotransferrin in capillary electrophoresis assays.
This a truncated version of the 80-mer aptamer developed by Tran et al. It was found to have a comparable binding affinity to the 80-mer version by Zou et al. Further truncations to 50- and 40-nucleotides reduced the binding affinity to ~15 nM.
Lysozyme (Apt 60) (ID# 457)
5'dApdGpdCpdApdGp dCpdApdCpdApdGp dApdGpdGpdTpdCp dApdGpdApdTpdGp dGpdCpdApdGpdGp dTpdApdApdGpdCp dApdGpdGpdCpdGp dGpdCpdTpdCpdAp dCpdApdApdApdAp dCpdCpdApdTpdTp dCpdGpdCpdApdTp dGpdCpdGpdGpdCp 3'
64
18587.09 g/mole
590400 L/(mole·cm)
58.33%
1.69
31.48
Tran DT, et al. (2010) Selection and characterization of DNA aptamers for egg white lysozyme. Molecules 15: 1127 - 1140.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Tran DT, et al. (2010) Selection and characterization of DNA aptamers for egg white lysozyme. Molecules 15: 1127 - 1140.
Zou M et al. (2012) The homogeneous fluorescence anisotropic sensing of salivary lysozyme using the 6-carboxyfluorescein-labeled DNA aptamer. Biosens. Bioelectron. 32: 148-154.
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