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Apta-Index™: Lysozyme (Apt 80) (ID# 456)

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Lysozyme (Apt 80) (ID# 456)

Lysozyme (Apt 80)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Hen egg white lysozyme and recombinant human lysozyme Protein 2.8 nM (reported value) Binding Buffer: 25 mM trizma base, 192 mM glycine, and 5 mM K2HPO4, pH 8.3. N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. This is the original 80-mer aptamer developed by Tran et al. against hen egg white lysozyme. See also the 60-mer truncated version (Apt 60) in the related aptamer section.

Zou et al. used fluorescence anisotrophy to measure standard recovery of recombinant human lysozyme (expressed from rice). Their results were comparable to a human lysozyme ELISA kit.
5'-dApdGpdCpdApdGp dCpdApdCpdApdGp dApdGpdGpdTpdCp dApdGpdApdTpdGp dGpdCpdApdGpdGp dTpdApdApdGpdCp dApdGpdGpdCpdGp dGpdCpdTpdCpdAp dCpdApdApdApdAp dCpdCpdApdTpdTp dCpdGpdCpdApdTp dGpdCpdGpdGpdCp dCpdCpdTpdApdTp dGpdCpdGpdTpdGp dCpdTpdApdCpdCp dGpdTpdGpdApdAp -3'
80 24742.06 g/mole 777200.00 L/(mole·cm) 57.50% 1.29 31.83

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Tran DT, et al. (2010) Selection and characterization of DNA aptamers for egg white lysozyme. Molecules 15: 1127 - 1140.

Zou M et al. (2012) The homogeneous fluorescence anisotropic sensing of salivary lysozyme using the 6-carboxyfluorescein-labled DNA aptamer. Biosens. Bioelectron. 32: 148-154.

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