Aptagen, LLC
RNA
C-Reactive Protein (CRP)
Protein
2.25 nM (reported value)
Binding Buffer: 20 mM Tris-HCl (pH 7.5), 140 mM NaCl, 2 mM CaCl2 and 1 mM MgCl2.
Incubation Time: 30 min. RT°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Binding experiments indicate that CaCl2 is necessary for binding, suggesting that the aptamer targets the pentamer form of CRP or that the aptamer itself requires CaCl2 to bind.
C-Reactive Protein (CRP1-1) (ID# 453)
Incubation Time: 30 min. RT°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Binding experiments indicate that CaCl2 is necessary for binding, suggesting that the aptamer targets the pentamer form of CRP or that the aptamer itself requires CaCl2 to bind.
5'rGprGprGp rCprGprAp rAprUprUp rCprGprGp rGprAprCp rUprUprCp rGprAprUp rCprCprGp rUprAprGp rUprAprCp rCprCprAp rCprCprAp rGprGprCp rAprUprAp rCprAprCp rCprAprGp rCprAprCp rGprCprGp rGprAprGp rCprCprAp rAprGprGp rAprAprAp rAprAprUp rAprGprUp rAprAprAp rCprUprAp rGprCprAp rCprUprCp rAprGprUp rGprCprUp rCprGprUp rAprUprGp rCprGprGp rAprAprGp rCprUp3'
115
33559.36 g/mole
1033300 L/(mole·cm)
53.85%
0.97
32.48
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
N Orito, Y Kikuchi, et al. High-affinity RNA aptamers to C-reactive protein (CRP): newly developed pre-elution methods for aptamer selection. J. Phys. Conf. Ser. 352(2012): 012042. doi:10.1088/1742-6596/352/1/012042.
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