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Apta-Index™: CCRF-CEM (sgc8c) (ID# 448)

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CCRF-CEM (sgc8c) (ID# 448)

CCRF-CEM (sgc8c)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Acute lymphoblastic leukemia (CCRF-CEM) Cells 0.78 nM (reported value) Binding buffer: 0.1mg/ml yeast tRNA and 1 mg/ml BSA into wash buffer (4.5g/L glucose, 5 mM MgCl2 in Dulbecco's PBS with CaCl2 and MgCl2) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. This aptamer is a truncated version of sgc8.
Huang et al. (2009) conjugated doxorubicin to sgc8c through a hydrazone linker. The sgc8c-doxorubicin conjugate was internalized through receptor-mediated uptake and doxorubicin was released in the endosome. This lead to an increase in toxicity to CCRF-CEM cells of 6.7-fold compared to NB-4 cells. The binding affinity (Kd) of the conjugate was comparable to the unconjugated sgc8c aptamer (2.0 nM).
5'-dApdTpdCp dTpdApdAp dCpdTpdGp dCpdTpdGp dCpdGpdCp dCpdGpdCp dCpdGpdGp dGpdApdAp dApdApdTp dApdCpdTp dGpdTpdAp dCpdGpdGp dTpdTpdAp dGpdAp -3'
41 12634.26 g/mole 397600.00 L/(mole·cm) 51.22% 2.52 31.78

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Sequence:
Shangguan et al. "Optimization and Modifications of Aptamers Selected from Live Cancer Cell Lines." Chem. Biochem., 8, (2007): 603-6.

Doxorubicin conjugation:
Y-F Huang et al. Molecular assembly of an aptamer-drug conjugate for targeted drug delivery to tumor cells. Chembiochem. 10(2009):862-868.

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