Aptagen, LLC
DNA
Thrombin
Protein
0.35 nM (reported value)
Binding Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM KCl, pH 8.0.
25°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
This aptamer is a dimerized version of the 15-mer and 29-mer thrombin aptamers connected by a 20-mer thymidine linker. This aptamer showed improved affinity and inhibitory effects to the monomer components.
Thrombin (15mer-29-mer dimer with 20T linker) (ID# 447)
5'dGpdGpdTp dTpdGpdGp dTpdGpdTp dGpdGpdTp dTpdGpdGp dTpdTpdTpdTpdTp dTpdTpdTpdTpdTp dTpdTpdTpdTpdTp dTpdTpdTpdTpdTp dApdGpdTp dCpdCpdGp dTpdGpdGp dTpdApdGp dGpdGpdCp dApdGpdGp dTpdTpdGp dGpdGpdGp dTpdGpdAp dCpdTp3'
70
19958.03 g/mole
588600 L/(mole·cm)
42.19%
1.70
33.91
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
H. Hasegawa et al. Improvement of aptamer affinity by dimerization. Sensors 8(2008): 1090-1098.
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