Aptamer Details

Thrombin (15mer-29-mer dimer with 20T linker) (ID# 447)

Thrombin (15mer-29-mer dimer with 20T linker)
DNA Thrombin Protein 0.35 nM (reported value) Binding Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM KCl, pH 8.0. 25°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. This aptamer is a dimerized version of the 15-mer and 29-mer thrombin aptamers connected by a 20-mer thymidine linker. This aptamer showed improved affinity and inhibitory effects to the monomer components.
5'dGpdGpdTp dTpdGpdGp dTpdGpdTp dGpdGpdTp dTpdGpdGp dTpdTpdTpdTpdTp dTpdTpdTpdTpdTp dTpdTpdTpdTpdTp dTpdTpdTpdTpdTp dApdGpdTp dCpdCpdGp dTpdGpdGp dTpdApdGp dGpdGpdCp dApdGpdGp dTpdTpdGp dGpdGpdGp dTpdGpdAp dCpdTp3' 70 19958.03 g/mole 588600 L/(mole·cm) 42.19% 1.70 33.91

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

H. Hasegawa et al. Improvement of aptamer affinity by dimerization. Sensors 8(2008): 1090-1098.

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