Forget Antibodies. Use Aptamers!™ Forget Antibodies. Use Aptamers!™
R&D Inquiry Quote
Contact Us Telecon Calls

Apta-Index™: Neuropeptide Y (DP3) (ID# 441)

Back to Search Results

Neuropeptide Y (DP3) (ID# 441)

Neuropeptide Y (DP3)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
2'-NH2-RNA Neuropeptide Y (NPY) Peptide 370 nM (reported value) Binding Buffer: 0.9 mM KH2P4, Na2HPO4, 70 mM NaCL, 1.3 mM KCl, 1% heparin, 0.4 g/l of streptavidin, pH 7.6. N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Truncation of the target peptide showed that this aptamer targets the C-terminus of neuropeptide Y.
Competition assays showed that this aptamer inhibits binding of neuropeptide Y to the Y2 receptor (IC50 of 73 nM). Less inhibition was observed for the Y1 and Y5 receptors (IC50 of 328 nM and 416 nM, respectively).
5'-rGprGprGprAprGprAprAprAprGprGprGprAprAprGpnCpnUpnUprGprAprGp nCprAprGpnCprAprGprGprAprGprGprGpnCpnCprGprGpnCprGpnUpnUprAprGprGprGpnUpnUprAprGpnCprGprAprGpnCpnCprGprApnUpnUprGprAprAp rAprGprAprAprGprAprAprGprGprAprApnCprGprAprGpnCprGpnUprApnCprGprGprApnUpnCpnCprGprApnUpnCp -3'
90 45033.34 g/mole 928300.00 L/(mole·cm) 57.78% 1.08 48.51

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

D. Proske et al. A Y2 receptor mimetic aptamer directed against neuropeptide Y. J. Biol. Chem. 277(2002):11416-11422.

Have your aptamer oligo synthesized by one of our AFFILIATES

Community Feedback

Aptagen, LLC | 250 North Main Street | Jacobus, PA 17407

Forget Antibodies. Use Aptamers!™ Forget Antibodies. Use Aptamers!™
Contact Us Telecon Calls