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Apta-Index™: Heparanase (1.5M NaCl) (ID# 425)

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Heparanase (1.5M NaCl) (ID# 425)

Heparanase (1.5M NaCl)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Heparanase (HPSE1) Protein 12 nM nM (reported value) Salt Solution: 100 mM NaCl, 20 mM KCl, 5 mM MgCl2 N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Selected in the presence of 1.5 M NaCl.
This aptamer showed significant inhibition of heparanase in a Matrigel cell invasion assay, outperforming the polyclonal anti-herparanase antibody used as a positive control.
A 30-nt truncated version of this aptamer including only the stem-loop structure showed similar binding affinity but diminished inhibitory activity.
5'-dGpdGpdGpdApdGpdApdCpdApdApdGpdApdApdTpdApdApdApdCpdGpdCpdTpdCpdApdAp dApdTpdGpdGpdApdCpdTpdTpdTpdTpdGpdApdApdTpdGpdTpdGpdGpdCpdApdApdCpdApdApdAp dTpdTpdCpdGpdApdCpdApdGpdGpdApdGpdGpdCpdTpdCpdApdCpdApdApdCpdApdGpdGpdCp -3'
72 22348.62 g/mole 727000.00 L/(mole·cm) 45.83% 1.38 30.74

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Simmons, SC, et al. Development of novel single-stranded nucleic acid aptamers against the pro-angiogenic and metastatic enzyme heparanase (HPSE1). PLoS One (2012) 7:e37938.

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