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Apta-Index™: Thrombin (31mer) (D2-5) (ID# 422)

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Thrombin (31mer) (D2-5) (ID# 422)

Thrombin (31mer) (D2-5)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Thrombin Protein N/A nM (reported value) Buffer for Clotting Inhibition assay: Tris-HCl pH 8.0, 5 mM KCl, 100 mM NaCl. N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Aptamer consists of a stem-loop (see figure) with the loop forming a G-quartet identical to the 15-mer thrombin-binding aptamer in the Related Aptamers section below. The authors optimized the stem for clotting inhibition.
5'-dApdGpdCpdGpdAp dGpdTpdApdGpdGpdTpdTpdGpdGpdTpdGpdTpdGpdGpdTpdTpdGpdGpdGpdGpdCp dTpdCpdGpdCpdTp-3'
31 9710.34 g/mole 295700.00 L/(mole·cm) 61.29% 3.38 32.84

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

K Ikebukuro et al. Analysis of the evolution of the thrombin-inhibiting DNA aptamers using a genetic algorithm. Biotechnol Lett (2006) 28:1933–1937.

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