Aptagen, LLC
DNA
Thrombin
Protein
N/A nM (reported value)
Buffer for Clotting Inhibition assay: Tris-HCl pH 8.0, 5 mM KCl, 100 mM NaCl.
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Aptamer consists of a stem-loop (see figure) with the loop forming a G-quartet identical to the 15-mer thrombin-binding aptamer in the Related Aptamers section below. The authors optimized the stem for clotting inhibition.
Thrombin (31mer) (D2-5) (ID# 422)
5'dApdGpdCpdGpdAp dGpdTpdApdGpdGpdTpdTpdGpdGpdTpdGpdTpdGpdGpdTpdTpdGpdGpdGpdGpdCp dTpdCpdGpdCpdTp3'
31
9710.34 g/mole
295700 L/(mole·cm)
61.29%
3.38
32.84
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
K Ikebukuro et al. Analysis of the evolution of the thrombin-inhibiting DNA aptamers using a genetic algorithm. Biotechnol Lett (2006) 28:1933ƒ??1937.
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