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Apta-Index™: RBP4 binding aptamer (RBA) (ID# 408)

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RBP4 binding aptamer (RBA) (ID# 408)

RBP4 binding aptamer (RBA)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Retinol-binding protein-4 (RBP4) Protein 201 nM (reported value) Binding Buffer: 100 mM NaCl, 25 mM Tris-HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, and 0.02% Tween-20, pH 7.6. N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Limit of detection using ELAAS assay was 78 ng/ml. See SJ Lee et al (2012) for more details.
5'-dApdTpdApdCpdCpdApdGpdCpdTpdTp dApdTpdTpdCpdApdApdTpdTpdApdCp dApdGpdTpdApdGpdTpdGpdApdGpdGp dGpdGpdTpdCpdCpdGpdTpdCpdGpdTp dGpdGpdGpdGpdTpdApdGpdTpdTpdGp dGpdGpdTpdCpdGpdTpdGpdGpdApdGp dApdTpdApdGpdTpdApdApdGpdTpdGp dCpdApdApdTpdCpdTp-3'
76 23679.45 g/mole 755800.00 L/(mole·cm) 47.37% 1.32 31.33

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

SJ Lee et al. Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays. Sensors Actuat. B-Chem.(2012): 243-248.

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