Aptagen, LLC
DNA
HER2 peptide
Protein
18.9 nM (reported value)
Hanks buffer with 0.1 mg/mL salmon sperm DNA and 1 mg/mL of BSA
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Targets HER2-expressing cell lines such as SK-BR-3.
Does not bind to HER2-negative cell lines MDA-MB-231 and MCF-7. Aptamer was complexed with doxorubicin and selectively delivered doxorubicin to HER2-positive cells in vitro.
HER-2 (HB5) (ID# 380)
Does not bind to HER2-negative cell lines MDA-MB-231 and MCF-7. Aptamer was complexed with doxorubicin and selectively delivered doxorubicin to HER2-positive cells in vitro.
5' dApdApdCpdCpdGpdCpdCpdCpdApdAp dApdTpdCpdCpdCpdTpdApdApdGpdAp dGpdTpdCpdTpdGpdCpdApdCpdTpdTp dGpdTpdCpdApdTpdTpdTpdTpdGpdTp dApdTpdApdTpdGpdTpdApdTpdTpdTp dGpdGpdTpdTpdTpdTpdTpdGpdGpdCp dTpdCpdTpdCpdApdCpdApdGpdApdCp dApdCpdApdCpdTpdApdCpdApdCpdAp dCpdGpdCpdApdCpdAp3'
89
26271.12 g/mole
811300 L/(mole·cm)
43.02%
1.23
32.38
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Z Liu et al. Novel HER2 aptamer selectively delivers cytotoxic drug to HER2-positive breast cancer cells in vitro. J Transl Med 10(2012): 148-157.
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