Aptamer Details

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V7t1 targeting VEGF-165 (ID# 368)

Aptamer Chemistry: DNA Target: VEGF-165 Antigen/Target Category: Protein Affinity (Kd): 1.4 nM (reported value) Binding Conditions/Buffer: 10% (v/v) human serum in TBSTE (TBSE containing 0.05% (v/v) of Tween-20). Binding Temp: 24°C Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Specificity: V7t1 is a truncated DNA aptamer that targets both VEGF-121 and VEGF-165. Comments:

Sequence (): 5'dTpdGpdTpdGpdGpdGpdGpdGpdTpdGpdGpdApdCpdGpdGpdGpdCpdCpdGpdGpdGpdTpdApdGpdAp3' Length: 25 Molecular Weight: 7900.16 g/mole Extinction Coefficient: 248000 L/(mole·cm) GC Content: 72.00% nmoles/OD₂₆₀: 4.03 μg/OD₂₆₀: 31.86

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Nonaka,Y., Sode, K., and Ikebukuro, K.(2010) "Screening and Improvement of an Anti-VEGF DNA Aptamer." Molecules, 15, 215-225.
Stejskalov?, Anna & Oliva, Nuria & J England, Frances & Almquist, Benjamin. (2019). Biologically Inspired, Cell-Selective Release of Aptamer-Trapped Growth Factors by Traction Forces. Advanced Materials. 1806380. 10.1002/adma.201806380.

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Aptamer Details

V7t1 targeting VEGF-165 (ID# 368)

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