Aptagen, LLC
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
2'-F-RNA
HIV-1 R5 gp120
Protein
31 nM (reported value)
cHBS buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM CaCl 2, 1 mM MgCl2, 2.7 mM KCl
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
IC= ~2nM in HIV-1Ba-L. This aptamer is a truncated version of B40.
HIV-1 R5 SU Glycoprotein gp120 (B40t77) (ID# 366)
5' rGprGprGprAprGprApfCprAprAprGp rApfCpfUprAprGprApfCprGpfCpfUp fCprAprApfUprGpfUprGprGprGpfCp fCprApfCprGpfCpfCpfCprGprApfUp fUpfUpfUprApfCprGpfCpfUpfUpfUp fUprApfCpfCpfCprGpfCprApfCprGp fCprGprApfUpfUprGprGpfUpfUpfUp rGpfUpfUpfUpfCpfCpfCp3'
84
24701.55 g/mole
737700 L/(mole·cm)
54.55%
1.36
33.48
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
A. K. Dey et. al. Structural characterization of an anti-gp120 RNA aptamer that neutralizes R5 strains of HIV-1. RNA 11:873-884
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