Aptagen, LLC
DNA
H4K16Ac
Peptide
47 nM (reported value)
Washed with buffer A (150 mM NaCl, 5 mM Na2HPO4, 1 mM EDTA, pH 7.5) and PBS buffer (150 mM NaCl, 5 mM Na2HPO4. pH 7.5) three times.
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Histone (H4K16Ac) (ID# 356)
5'dApdGpdApdCpdGpdTpdApdApdGpdTpdTpdApdApdTpdTpdGpdGpdApdCpdTpdTpdGpdGpdTpdCpdGpdTpdGpdTpdGpdCpdGpdGpdCpdApdCpdApdGpdCpdGpdApdTpdTpdGpdApdApdApdTp3'
48
14926.78 g/mole
476300 L/(mole·cm)
45.83%
2.10
31.34
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Lin et al. "Recognition Imaging of Acetylated Chromatin Using a DNA Aptamer." Biophysical Journal, 97(2009): 1804-07.
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