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Apta-Index™: PSA aptamer (PSap4#5) (ID# 332)

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PSA aptamer (PSap4#5) (ID# 332)

PSA aptamer (PSap4#5)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA PSA Protein <100 nM (reported value) 10 mM Tris/HCl, 150 mM NaCl, 5 mM KCl, 5 mM MgCl2, 0.05% Tween 20, pH 7.4, r.t. The DNA library was heated at 95 °C for 10 min and then gradually cooled to 25 °C over a period of 30 min in TBS buffer (10mM Tris/HCl, 150mM NaCl, 5mM KCl, 5mM MgCl2, pH 7.4). Binding was not detectable during the course of internal investigation. Gel-Shift and Forte-Bio Octet methods to attempt to determine Kd did not generate results indicative of aptamer-target binding.
5'-dTpdTpdTpdTpdTpdApdApdTpdTpdApdApdApdGpdCpdTpdCpdGpdCpdCpdApdTpdCpdApdApdApdTpdApdGpdCpdTpdTpdTp-3'
32 9747.45 g/mole 305200.00 L/(mole·cm) 28.13% 3.28 31.94

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

N Savory et al. Selection of DNA aptamer against prostate specific antigen using a genetic algorithm and application to sensing. Biosens. Bioelectron. 26 (2010): 1386-1391.

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