Aptamer Details

Chloramphenicol-binding aptamer(c7) (ID# 329)

Aptamer Chemistry: DNA Target: chloramphenicol (cam) Antigen/Target Category: Small Organic Affinity (Kd): 0.766 µM (reported value) Binding Conditions/Buffer: 100mM NaCl, 20 mM Tris-HCl, 2 mM MgCl₂, 5 mM KCl, 1 mM CaCl₂, 0.02 Tween 20, pH 7.6, r.t. Binding Temp: N/A°C Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Comments:

Sequence (): 5'dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdApdCpdTpdTpdCpdApdGpdTpdGpdApdGpdTpdTpdGpdTpdCpdCpdCpdApdCpdGpdGpdTpdCpdGpdGpdCpdGpdApdGpdTpdCpdGpdGpdTpdGpdGpdTpdApdGpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTp dApdCpdCpdGpdTpdGpdApdAp3' Length: 80 Molecular Weight: 24817.1 g/mole Extinction Coefficient: 774600 L/(mole·cm) GC Content: 57.50% nmoles/OD₂₆₀: 1.29 μg/OD₂₆₀: 32.04

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

J Mehta et al. In vitro selection and characterization of DNA aptamers recognizing chloramphenicol. J. Biotechnol. 155(2011): 361-369.

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Aptamer Details

Chloramphenicol-binding aptamer(c7) (ID# 329)

J Mehta et al. In vitro selection and characterization of DNA aptamers recognizing chloramphenicol. J. Biotechnol. 155(2011): 361-369.

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