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Apta-Index™: Chloramphenicol-binding aptamer(c7) (ID# 329)

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Chloramphenicol-binding aptamer(c7) (ID# 329)

Chloramphenicol-binding aptamer(c7)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA chloramphenicol (cam) Small Organic 0.766 µM (reported value) 100mM NaCl, 20 mM Tris-HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02 Tween 20, pH 7.6, r.t. N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdApdCpdTpdTpdCpdApdGpdTpdGpdApdGpdTpdTpdGpdTpdCpdCpdCpdApdCpdGpdGpdTpdCpdGpdGpdCpdGpdApdGpdTpdCpdGpdGpdTpdGpdGpdTpdApdGpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTp dApdCpdCpdGpdTpdGpdApdAp-3'
80 24817.10 g/mole 774600.00 L/(mole·cm) 57.50% 1.29 32.04

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

J Mehta et al. In vitro selection and characterization of DNA aptamers recognizing chloramphenicol. J. Biotechnol. 155(2011): 361-369.

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