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Apta-Index™: Mercury II (Hg2+) (ID# 325)

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Mercury II (Hg2+) (ID# 325)

Mercury II (Hg2+)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Mercury II (Hg2+) Other 40 nM (reported value) 3-(N-morpholino)propanesulfonic acid (10mM, pH 7.0, NaCl (25mM), NaNO3 (500mM), and ethylenediamine (0.1 mM) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Authors attached dabcyl (quencher) and 6-fluorescein (fluorophore) to the 5' and 3' ends of the aptamer. In the presence of Hg2+ ions, the aptamer forms a hairpin with T-Hg2+-T bonds. This brings the quencher into proximity of the fluorophore.
5'-dTpdTpdCpdTpdTpdTpdCpdTpdTpdCpdCpdCpdCpdTpdTpdGpdTpdTpdTpdGpdTpdTp-3'
22 6590.34 g/mole 178600.00 L/(mole·cm) 36.36% 5.60 36.90

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

A. Ono and H. Togashi. "Highly selective oligonucleotide-based sensor for mercury(II) in aqueous solutions." Angew. Chem. Int. Ed. 43(2004):4300-4302.

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