Aptagen, LLC
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
2'-F-RNA
Integrin ??3 subunit
Protein
6.5 nM (reported value)
Binding Buffer: PBS supplemented with 0.05% Tween-20 and 0.1% BSA.
Incubation Time: 1 h.
Folding Protocol: Heated to 95°C for 5 min and then snap-colled on ice of 5 min.
Assay Type: ELONA RT°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. No binding was observed to integrin aV??6 at 250 nM, whereas the aptamer recognized integrin aV??3 and aIIb??3 at 100 pM. See also aptamer aV-1 which binds to the aV subunit.
Integrin ??3 subunit (??3-1) (ID# 321)
Incubation Time: 1 h.
Folding Protocol: Heated to 95°C for 5 min and then snap-colled on ice of 5 min.
Assay Type: ELONA RT°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. No binding was observed to integrin aV??6 at 250 nM, whereas the aptamer recognized integrin aV??3 and aIIb??3 at 100 pM. See also aptamer aV-1 which binds to the aV subunit.
5'rGprGprGprAprGprGprApfCprGprApfUprGpfCprGprGpfCpfCpfCprAprGprApfUpfUprApfCpfUprGpfUprGprGprAprGpfUprGprGpfUpfUprGpfUpfCpfUprGpfCprGprAprApfUpfCpfCpfUpfUpfCprGpfUpfCpfCprApfCpfCpfCprAprApfUprAprGpfCprAprGprApfCprGprApfCpfUpfCprGpfCpfCpfCprGprAp3'
81
26160.56 g/mole
784800 L/(mole·cm)
59.26%
1.27
33.33
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Gong, Q. et al,(2012). Selection strategy to generate aptamer pairs that bind to distinct sites on protein targets. Analytical Chemistry, 2012(84), 5365-5371.
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