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Apta-Index™: DNA Blocker (Part of Lysozyme Aptasensor) (ID# 319)

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DNA Blocker (Part of Lysozyme Aptasensor) (ID# 319)

DNA Blocker (Part of Lysozyme Aptasensor)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Lysozyme Other 0.1 fM (reported value) NEBuffer 2(10X): 50 mM NaCl, 10 mM Tris-HCL, 10 mM MgCl2 1 mM dithiothreitol, pH 7.9.

Detection Buffer (total volume): 25 mM HEPES, 20 mM KCL, 200 mM NaCl, 0.05% Triton X-100 and 1% DMSO; pH 7.4) with 0.5 μM hemin.

Consult ref. for procedure.
N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Contains a lysozyme-binding aptamer (capitalized in the structure) and a region complimentary to DNAzymeMB (highlighted; see related aptamers).
5'-dApdTpdCpdTpdApdCpdGpdApdApdTpdTpdCpdApdTpdCpdApdGpdGpdGpdCpdTpdApdApdApdGpdApdGpdTpdGpdCpdApdGpdApdGpdTpdTpdApdCpdTpdTpdApdGpdCpdCpdCpdGpdTpdGpdAp-3'
49 15128.91 g/mole 488200.00 L/(mole·cm) 44.90% 2.05 30.99

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Fu et al. "An ultrasensitive peroxidase DNAzyme-associated aptasensor that utilizes a target-triggered enzymatic signal amplification strategy." Chemical Communications, 47(2011): 9876-9878.

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