Aptagen, LLC
DNA
nontumorigenic HF cell line
Cells
1.6 nM (reported value)
1% BSA in PBS with salts
4°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Aptamer 20 has higher affinity than aptamer 14 against the HF cell line but does not internalize.
HPV-HF cell line (20) (ID# 317)
5'dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdGpdGpdGpdGpdApdGpdGpdGpdApdGpdApdCpdApdCpdApdGpdTpdCpdApdTpdGpdGpdApdGpdCpdApdGpdTpdTpdApdTpdTpdApdGpdGpdGpdTpdGpdTpdApdCpdCpdGpdGpdGpdTpdGpdTpdApdGpdTpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp3'
87
27123.72 g/mole
880300 L/(mole·cm)
44.83%
1.14
30.81
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Graham JC, Zarbl H (2012) Use of Cell-SELEX to Generate DNA Aptamers as Molecular Probes of HPV-Associated Cervical Cancer Cells. PLoS ONE 7(4): e36103. doi:10.1371/journal.pone.0036103
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