Aptagen, LLC
RNA
G protein-coupled receptor kinase 2
Protein
1.2 nM (reported value)
Binding Buffer 20 mM Tris (pH 7.0), 50 mM NaCl, 5 mM MgCl2,2 mM DTT, and 0.1% Lubrol (without nonspecific RNA)
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Activity IC50 - 11 nM Binding IC50 - 1.5 nM
GRK2 (C13.28) (ID# 314)
5'rGprGprCprAprGprAprCprCprAprUprAprCprGprGprGprAprGprAprGprAprAprAprCprUprUprGprCprCp3'
28
9062.59 g/mole
282300 L/(mole·cm)
57.14%
3.54
32.10
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Tesmer et al., Molecular Mechanism for Inhibition of G Protein-Coupled Receptor Kinase 2 by a Selective RNA Aptamer, Structure (2012), doi:10.1016/j.str.2012.05.002
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