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Plasmodium falciparum lactate dehydrogenase (pL1) (ID# 309)

Aptamer Chemistry: DNA Target: Plasmodium falciparum lactate dehydrogenase (PfLDH) Antigen/Target Category: Protein Affinity (Kd): 38.7 nM (reported value) Binding Conditions/Buffer: Binding Buffer: 20mM Trisƒ??HCl pH 8.0, 50mM NaCl, 5mM KCl, and 5mM MgCl₂) Binding Temp: RT°C Refolding Protocol: Denatured at 90°C for 3 min and then incubated at 4°C for 1 h. Comments: For details on pL1's use in colorimetric testing, see: Lee S, Manjunatha DH, Jeon W, Ban C (2014) Cationic Surfactant-Based Colorimetric Detection of Plasmodium Lactate Dehydrogenase, a Biomarker for Malaria, Using the Specific DNA Aptamer. PLoS ONE 9(7): e100847. doi:10.1371/journal.pone.0100847

Sequence (): 5'dGpdTpdTpdCpdGpdApdTpdTpdGpdGpdApdTpdTpdGpdTpdGpdCpdCpdGpdGpdApdApdGpdTpdGpdCpdTpdGpdGpdCpdTpdCpdGpdApdApdCp3' Length: 36 Molecular Weight: 11163.29 g/mole Extinction Coefficient: 340000 L/(mole·cm) GC Content: 55.56% nmoles/OD₂₆₀: 2.94 μg/OD₂₆₀: 32.83

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Seonghwan, L. (2012). A highly sensitive aptasensor towards plasmodium lactate dehydrogenase for the diagnosis of malaria. Biosensors and Bioelectronics, 35(2012), 291-296.

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Aptamer Details

Plasmodium falciparum lactate dehydrogenase (pL1) (ID# 309)

Seonghwan, L. (2012). A highly sensitive aptasensor towards plasmodium lactate dehydrogenase for the diagnosis of malaria. Biosensors and Bioelectronics, 35(2012), 291-296.

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