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Apta-Index™: Interleukin 10 (IL-10) (R5A1) (ID# 291)

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Interleukin 10 (IL-10) (R5A1) (ID# 291)

Interleukin 10 (IL-10) (R5A1)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Interleukin -10 (IL-10) Protein 12 nM (reported value) 150 mmol/L NaCl, 20 mmol/L KCl, and 2 mmol/L HEPES heated to 82°C and slowly cooling to room temperature N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Aptamer was truncated and modified with 2'F and 2'O-methyl, but no KD's were given for these adaptations.
5'-rGprGprGp rCprUprCp rAprUprGp rCprAprCp rGprUprUp rUprGprCp rUprCprCp rUprGprUp rAprAprUp rUprGprGp rCprGprUp rAprUprGp rUprAprAp rCprCprCp rAprGprGp rCprAprCp rCprAprAp rAprCprAp rCprCprCp rCprAprGp rGprCprCp rGprGprGp rCprCprAp rUprGprAp rUprCprCp rAprCprAp rUprAp-3'
80 25585.42 g/mole 765600.00 L/(mole·cm) 56.25% 1.31 33.42

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Berezhnoy, A, et al. (2012). Isolation and optimization of murine il-10 receptor blocking oligonucleotide aptamers using high- throughput sequencing. The American Society of Gene & Cell Therapy

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