Aptagen, LLC
DNA
Nucleolin
Protein
N/A nM (reported value)
For fluoresecent labeling, 400 nM aptamer were added to cell growth media for 1 hour at 37°C and 5% CO2. Dulbecco's phosphate-buffered saline was used to wash cells.
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
The image does not reflect the G quartet structure.
Kotula's et al. research suggests that the aptamer is internalized through a nonstandard mechanism.
Nucleolin aptamer (AS1411) (ID# 290)
Kotula's et al. research suggests that the aptamer is internalized through a nonstandard mechanism.
5'dGpdGpdTp dGpdGpdTp dGpdGpdTp dGpdGpdTp dTpdGpdTp dGpdGpdTp dGpdGpdTp dGpdGpdTp dGpdGp3'
28
8272.41 g/mole
250800 L/(mole·cm)
65.38%
3.99
32.98
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Kotula J, Sullenger B, et al. 2012. Aptamer-mediated delivery of splice-switching oligonucleotides to the nuclei of cancer cells. Nucleic Acid and Therapeutics 22.3:187-195. Li, L. et al. 2014. Aptamer photoregulation in vivo. PNAS 11.48: 17099-103.
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