Aptagen, LLC
RNA
HER-2 (SK-BR-3 cell line)
Cells
94.6 nM (reported value)
Binding buffer: 4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, 1 mg/mL BSA in Dulbeccoƒ??s PBS.
Washing buffer: 4.5 g/L glucose, 5 mM MgCl2 in Dulbeccoƒ??s PBS 4°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Binds to the breast cancer cell line SK-BR-3 which overexpresses Her-2 protein.
Does not bind to MDA-MB-231 which under expresses Her-2.
HER-2 (S6) (ID# 284)
Washing buffer: 4.5 g/L glucose, 5 mM MgCl2 in Dulbeccoƒ??s PBS 4°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Binds to the breast cancer cell line SK-BR-3 which overexpresses Her-2 protein.
Does not bind to MDA-MB-231 which under expresses Her-2.
5'rGprGprGprAprGprAprUprAprCprCprAprGprCprUprUprAprUprUprCprAprAprUprUprUprGprGprAprUprGprGprGprGprAprGprAprUprCprCprGprUprUprGprAprGprUprAprAprGprCprGprGprGprCprGprUprGprUprCprUprCprUprCprUprGprCprCprGprCprCprUprUprGprCprUprAprUprGprGprGprGprAprGprAprUprAprGprUprAprAprGprUprGprCprAprAprUprCprUp3'
98
31658 g/mole
976700 L/(mole·cm)
51.02%
1.02
32.41
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Kang, H. (2009). Isolation of RNA aptamers targeting Her-2-overexpressing breast cancer cells using cell-selex. Bull. Korean Chem. Soc., 30(8), 182-1831.
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