* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA HER-2 (SK-BR-3 cell line) Cells 94.6 nM (reported value)Binding buffer: 4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, 1 mg/mL BSA in Dulbecco’s PBS.
Washing buffer: 4.5 g/L glucose, 5 mM MgCl2 in Dulbecco’s PBSN/A
If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Kang, H. (2009). Isolation of RNA aptamers targeting Her-2-overexpressing breast cancer cells using cell-selex. Bull. Korean Chem. Soc., 30(8), 182-1831.
Have your aptamer oligo synthesized by one of our AFFILIATES
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA
HER-2 (SK-BR-3 cell line)
Cells
94.6 nM (reported value)
Binding buffer: 4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/mL yeast tRNA, 1 mg/mL BSA in Dulbecco’s PBS. 
