Aptagen, LLC
DNA
MPT64 Protein
Protein
N/A nM (reported value)
SHCMK binding buffer: 20 mM HEPES, pH 7.35, 120 mM KCL, 1 mM CaCl2, and 1 mM MgCl2
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Was used in a sandwich assay with M64CA.
M64CA (ID# 282)
5' dTpdTpdCpdGpdGpdGpdApdApdTpdGp dApdTpdTpdApdTpdCpdApdApdApdTp dTpdTpdApdTpdGpdCpdCpdCpdTpdCp dTpdGpdApdTp3'
35
10421.87 g/mole
326100 L/(mole·cm)
35.29%
3.07
31.96
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Qin, L., et al.(2009). "The selection and application of ssDNA aptamers against MPT64 protein in Mycobacterium tuberculosis." Clin Chem Lab Med, 47(4), 405-411. doi: 10.1515/CCLM.2009.097
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