* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA IgE (human) Protein 3.6 nM (reported value)PBS containing 1 mM MgCl2 (pH 7.4)N/A
If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.Aptamer binding was improved by Liss et al by extending the stem loop. The original aptamer lacked the last four base pairs in the stem loop (Kd = 8.4 nM).
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Aptamer Selection Reference: Wiegand, T.W., et al. "High-Affinity Oligonucleotide Ligands to Human IgE Inhibit Binding to FCE Receptor I." Journal of Immunology, 157 (1996): 221-230
Liss, M., et al. "An Aptamer-Based Quartz Crystal Protein Biosensor." Anal. Chem.,74 (2002): 4488-4495.
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* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA
IgE (human)
Protein
3.6 nM (reported value)
PBS containing 1 mM MgCl2 (pH 7.4)
N/A
If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Aptamer binding was improved by Liss et al by extending the stem loop. The original aptamer lacked the last four base pairs in the stem loop (Kd = 8.4 nM).
