Aptagen, LLC
DNA
Ramos Cells (B-cell lymphoma cell line)
Cells
74.7 nM (reported value)
Wash Buffer (4.5 g/L glucose and 5 mM MgCl2 in Dulbeccoƒ??s PBS). Binding Buffer (yeast tRNA (0.1 mg/mL) and BSA (1 mg/mL) into the wash buffer). On ice.
N/A°C
Denature by heating to 95° for 5 minutes. Then, cool on ice for 10 minutes.
TD05 was specific for Ramos cells relative to CCRF-CEM and Toledo cells
A modified/truncated version of TD05 exists: TD05.17. This version can be dimerized via a PEG linker to bind at physiological temperatures.
Ramos Cells (TD05) (ID# 273)
5'dApdApdCpdApdCpdCpdGpdTpdGpdGpdApdGpdGpdApdTpdApdGpdTpdTpdCpdGpdGpdTpdGpdGpdCpdTpdGpdTpdTpdCpdApdGpdGpdGpdTpdCpdTpdCpdCpdTpdCpdCpdCpdGpdGpdTpdGp3'
48
14847.64 g/mole
451600 L/(mole·cm)
60.42%
2.21
32.88
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Tang et al. "Selection of Aptamers for Molecular Recognition and Characterization of Cancer Cells." Analytical Chemistry, 79(2007): 4900-4907.
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