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Apta-Index™: Ramos Cells (TD05) (ID# 273)

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Ramos Cells (TD05) (ID# 273)

Ramos Cells (TD05)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Ramos Cells (B-cell lymphoma cell line) Cells 74.7 nM (reported value) Wash Buffer (4.5 g/L glucose and 5 mM MgCl2 in Dulbecco’s PBS). Binding Buffer (yeast tRNA (0.1 mg/mL) and BSA (1 mg/mL) into the wash buffer). On ice. Denature by heating to 95° for 5 minutes. Then, cool on ice for 10 minutes. A modified/truncated version of TD05 exists: TD05.17. This version can be dimerized via a PEG linker to bind at physiological temperatures.
5'-dApdApdCpdApdCpdCpdGpdTpdGpdGpdApdGpdGpdApdTpdApdGpdTpdTpdCpdGpdGpdTpdGpdGpdCpdTpdGpdTpdTpdCpdApdGpdGpdGpdTpdCpdTpdCpdCpdTpdCpdCpdCpdGpdGpdTpdGp-3'
48 14847.64 g/mole 451600.00 L/(mole·cm) 60.42% 2.21 32.88

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Tang et al. "Selection of Aptamers for Molecular Recognition and Characterization of Cancer Cells." Analytical Chemistry, 79(2007): 4900-4907.

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