Aptagen, LLC
DNA
Non-small Cell Lung Cancer
Cells
28.2 nM (reported value)
Wash Buffer (5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbeccoƒ??s PBS without calcium and magnesium) The binding buffer was prepared by adding 1 mg/mL bovine serum albumin (BSA) and 0.1 mg/mL yeast tRNA to washing buffer
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Non-small Cell Lung Cancer (S6) (ID# 271)
5'dApdCpdGpdCpdTpdCpdGpdGpdApdTpdGpdCpdCpdApdCpdTpdApdCpdApdGpdGpdTpdGpdGpdCpdCpdApdGpdTpdCpdApdCpdTpdCpdApdApdTpdTpdGpdGpdGpdTpdGpdTpdApdGpdGpdGpdGpdTpdGpdGpdGpdGpdApdTpdTpdGpdTpdGpdGpdGpdTpdTpdGpdCpdTpdCpdApdTpdGpdGpdApdCpdGpdTpdGpdCpdTpdGpdGpdTpdGpdApdCp3'
85
26491.17 g/mole
809200 L/(mole·cm)
58.82%
1.24
32.74
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Zhao et al. "Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells." Analyst 134(2009):1808-1814.
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