Aptagen, LLC
RNA
Unphosphorylated ERK2
Protein
2 nM (reported value)
34-37 °C, 10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 0.05 μg/μl tRNA
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Aptazyme using a hammerhead motif. See also the aptamer for phosphorylated ERK2. www.aptagen.com/customize_product.aspx?id=263
Unphosphorylated ERK2 (ID# 264)
5'rGprGprCp rGprUprGp rAprCprCp rUprGprAp rUprGprAp rGprUprCp rAprCprGp rCprUprAp rAprGprGp rAprGprGp rAprUprUp rUprCprCp rGprAprAp rAprGprCp rGprGprCp rUprAprCp rGprGprUp rCprCprGp rCprCprAp rGprUprGp rUprUprAp rCprGprAp rAprAprCp rGprUprUp rCprCprCp 3'
83
24170.59 g/mole
732700 L/(mole·cm)
57.33%
1.36
32.99
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Vaish et al. "Monitoring post-translational modifications of proteins with allosteric ribozymes." Nature Biotechnology, 20(2002): 810-815.
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