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Apta-Index™: Unphosphorylated ERK2 (ID# 264)

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Unphosphorylated ERK2 (ID# 264)

Unphosphorylated ERK2
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Unphosphorylated ERK2 Protein 2 nM (reported value) 34-37 °C, 10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 0.05 μg/μl tRNA N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Aptazyme using a hammerhead motif. See also the aptamer for phosphorylated ERK2.
5'-rGprGprCp rGprUprGp rAprCprCp rUprGprAp rUprGprAp rGprUprCp rAprCprGp rCprUprAp rAprGprGp rAprGprGp rAprUprUp rUprCprCp rGprAprAp rAprGprCp rGprGprCp rUprAprCp rGprGprUp rCprCprGp rCprCprAp rGprUprGp rUprUprAp rCprGprAp rAprAprCp rGprUprUp rCprCprCp -3'
75 24170.59 g/mole 732700.00 L/(mole·cm) 57.33% 1.36 32.99

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Vaish et al. "Monitoring post-translational modifications of proteins with allosteric ribozymes." Nature Biotechnology, 20(2002): 810-815.

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