Aptagen, LLC
RNA
Phosphorylated ERK2
Protein
N/A nM (reported value)
34-37 °C, 10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 0.05 µg/µl tRNA
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Contains hammerhead ribozyme motif. See also non-phosphorylated ERK2 aptamer. www.aptagen.com/customize_product.aspx?id=264
Phosphorylated ERK2 (ID# 263)
5'rGprGprCprGprUprGprAprCprCprUprGprAprUprGprAprGprUprCprAprCprGprCprAprGprAprCprGprCprUprAprGprCprGprAprAprUprUprGprGprUprUprCprCprUprCprGprAprAprAprGprGprGprGprAprAprAprGprCprGprUprUprAprUprUprAprAprGprAprAprAprCprCprAprAprAprAprUprGprUprGprUprUprAprCprGprAprAprAprCprGprUprUprCprCprCp3'
95
30687.59 g/mole
959300 L/(mole·cm)
48.42%
1.04
31.99
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Vaish et al. "Monitoring post-translational modifications of proteins with allosteric ribozymes." Nature Biotechnology, 20(2002): 810-815.
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