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Apta-Index™: Aspartame (ID# 261)

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Aspartame (ID# 261)

Aspartame
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Aspartame Protein 5 mM (reported value) Selection Buffer (150 mM NaCl, 10 mM MgCl2, 1 mM EDTA, 10 mM Sodium Phosphate, 90 mM HEPES (pH 7.1)) Assay Buffer (180mM HEPES (pH 7.0±8.0), 300 mM NaCl, 20 or 50 mM MgCl2, 2 mM EDTA, 20 mM Na phosphate), r.t. N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rGprGprAprUprGprUprCprCprAprGprUprCprGprCprUprUprGprCprAprAprUprGprCprCprCprUprUprUprUprAprGprAprCprCprCprUprGprAprUprGprAprGprCprGprGprUprGprCprUprAprGprUprUprAprGprUprUprGprCprAprGprUprUprUprCprGprGprUprUprGprUprUprAprCprGprCprGprAprAprAprCprGprGprUprGprAprAprAprGprCprCprGprUprAprGprGprUprCprUp-3'
99 31837.04 g/mole 970700.00 L/(mole·cm) 51.52% 1.03 32.80

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Ferguson et al. "A novel strategy for selection of allosteric ribozymes yields RiboReporter TM sensors for caffeine and aspartame." Nucleic Acids Research, 32(2004): 1756-1766.

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