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Apta-Index™: Fibrinogen (85A/Ap90) (ID# 215)

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Fibrinogen (85A/Ap90) (ID# 215)

Fibrinogen (85A/Ap90)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Fibrinogen Protein 63.8 nM (reported value) Binding Buffer: 300 mM NaCl, 5 mM MgCl2, 20 mM Tris-HCl at pH 7.6 N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Kd = 6.4 nM if thymidines are boronic acid modified.
5'-dCpdCpdTpdTpdCpdGpdTpdTpdGpdTpdCpdTpdGpdCpdCpdTpdTpdCpdGpdTpdApdGpdGpdApdCpdCpdGpdCpdApdGpdApdCpdApdTpdCpdGpdApdCpdGpdCpdApdGpdGpdGpdApdApdApdTpdTpdCpdCpdGpdCpdApdApdGpdTpdCpdCpdApdGpdCpdCpdApdApdApdTpdGpdCpdCpdApdCpdCpdCpdTpdTpdCpdApdGpdApdApdTpdTpdCpdGpdCpdApdCpdCpdAp-3'
90 27523.83 g/mole 840700.00 L/(mole·cm) 55.56% 1.19 32.74

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Minyong Li, Na Lin, Zhen Huang, Lupei Du, Craig Altier, Hao Fang and Binghe Wang. "Selecting Aptamers for a Glycoprotein through the Incorporation of the Boronic Acid Moiety." J. Am. Chem. Soc., 2008, 130 (38), pp 12636–12638.

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