Aptagen, LLC
DNA
Lactobacillum acidophilius
Cells
13 nM (reported value)
Washed twice with binding buffer: 50 mM Tris-HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, and 1 mM MgCl2
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Most likely target is the S-layer protein expressed on the cell surface.
Lactobacillum acidophilius (hemag1P) (ID# 212)
5'dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdTpdApdGpdCpdCpdCpdTpdTpdCpdApdApdCpdApdTpdApdGpdTpdApdApdTpdApdTpdCpdTpdCpdTpdGpdCpdApdTpdTpdCpdTpdGpdTpdGpdTpdGpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdGpdTpdGpdApdAp3'
78
24010.64 g/mole
751500 L/(mole·cm)
47.44%
1.33
31.95
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Hamula et al. "Selection of Aptamers against Live Bacterial Cells." Analytical Chemistry, 80(2008): 7812-7819.
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