Aptagen, LLC
RNA
FMN
Small Organic
N/A nM (reported value)
Standard Reaction Conditions: 50 mM Tris-HCl, pH 7.5, 20 mM MgCl2
2X Gel Loading Buffer (equal volume used to quench reaction): 87 mM Tris base, 89 mM boric acid, 20% sucrose, 0.05% bromophenol blue, 0.05% xylene cyanol, 0.1% sodium dodecyl sulfate (SDS), 7.3 M urea, 1 mM EDTA. Additional 40 mM EDTA added. 23°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Flavin Mononucleotide (AR5) (ID# 204)
2X Gel Loading Buffer (equal volume used to quench reaction): 87 mM Tris base, 89 mM boric acid, 20% sucrose, 0.05% bromophenol blue, 0.05% xylene cyanol, 0.1% sodium dodecyl sulfate (SDS), 7.3 M urea, 1 mM EDTA. Additional 40 mM EDTA added. 23°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'rGprGprAprUprAprAprUprAprGprCprCprGprUprAprGprGprUprUprGprCprGprAprAprAprGprCprGprAprCprCprCprUprGprAprUprGprAprGp rCprCprUprUprAprGprGprAprUprAprUprGprCprAprUprGprAprUprGprCprAprGprAprAprGprGprAprCprGprUp rCprGprAprAprAprCprGprGprUprAprGprCprGprAprGprAprGprCprUprCp 3'
89
28595.37 g/mole
897000 L/(mole·cm)
52.27%
1.11
31.88
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Seetharaman et al. "Immobilized RNA switches for the analysis of complex chemical and biological mixtures." Nature Biotechnology, 19(2001): 336-341.
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