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Apta-Index™: L-tryptophan(Trp 70-727) (ID# 195)

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L-tryptophan(Trp 70-727) (ID# 195)

L-tryptophan(Trp 70-727)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA L-tryptophan Protein 15 nM (reported value) Selection buffer: 50 mM HEPES (pH 7.0), 250 mM NaCl, 5 mM MgCl2, 5 mM CaCl2, 5 mM glycine N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rGprGprGprAprUprCprCprUprAprAprGprCprUprCprUprAprUprCprGprGprCprUprGprGprAprCprGprAprCprGprGprGprGprAprCprGprCprCprAprCprUprGprGprAprCprUprAprGprGprUprAprAprGprCprCprAprGprGprAprCprCprGprUprAprCprGprUprCprGprGprGprAprGprCprCprGprUprCprAprGprAprAprUprAprAprAprAprGprCprGprGprCprCprUprAprGprCprGprAprUprCprGprAprUp-3'
104 33718.43 g/mole 1026700.00 L/(mole·cm) 59.62% 0.97 32.84

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Majerfeld and Yarus. "A diminutive and specific RNA binding site for L-tryptophan." Nucleic Acids Research, 33(2005): 5482-5493.

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