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Apta-Index™: Tetracycline (ID# 191)

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Tetracycline (ID# 191)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA Tetracycline Small Organic 63.6 nM (reported value) Binding Buffer: 100 mM NaCl, 20 mM Tris-HCl (pH 7.6), 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% Tween-20 N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Aptamer was immobilized on steptavidin-modified screen-printed gold electrodes to create a sensitive aptasensor for tetracycline (Kim et al. 2010). The range of detection was 0.01-10 µM.
5'-dCpdGpdTp dApdCpdGp dGpdApdAp dTpdTpdCp dGpdCpdTp dApdGpdCp dCpdCpdCp dCpdCpdGp dGpdCpdAp dGpdGpdCp dCpdApdCp dGpdGpdCp dTpdTpdGp dGpdGpdTp dTpdGpdGp dTpdCpdCp dCpdApdCp dTpdGpdCp dGpdCpdGp dTpdGpdGp dApdTpdCp dCpdGpdAp dGpdCpdTp dCpdCpdAp dCpdGpdTp dGp-3'
76 23367.05 g/mole 688400.00 L/(mole·cm) 68.42% 1.45 33.94

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Niazi JH, Lee SJ, Gu MB. (2008) Single-stranded DNA aptamers specific for antibiotics tetracyclines. Bioorg Med Chem. 16: 7245-7253.

Kim YJ, Kim YS, Niazi JH, Gu MB. (2010) Electrochemical aptasensor for tetracycline detection. Bioprocess Biosyst Eng. 33:31-37.

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