Aptagen, LLC
DNA
Tetracycline
Small Organic
63.6 nM (reported value)
Binding Buffer: 100 mM NaCl, 20 mM Tris-HCl (pH 7.6), 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% Tween-20
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Weakly binds to doxycycline and oxytetracycline.
Aptamer was immobilized on steptavidin-modified screen-printed gold electrodes to create a sensitive aptasensor for tetracycline (Kim et al. 2010). The range of detection was 0.01-10 µM.
Tetracycline (ID# 191)
5'dCpdGpdTpdApdCpdGpdGpdApdApdTpdTpdCpdGpdCpdTpdApdGpdCpdCpdCpdCpdCpdCpdGpdGpdCpdApdGpdGpdCpdCpdApdCpdGpdGpdCpdTpdTpdGpdGpdGpdTpdTpdGpdGpdTpdCpdCpdCpdApdCpdTpdGpdCpdGpdCpdGpdTpdGpdGpdApdTpdCpdCpdGpdApdGpdCpdTpdCpdCpdApdCpdGpdTpdGp3'
76
23367.05 g/mole
688400 L/(mole·cm)
68.42%
1.45
33.94
Niazi JH, Lee SJ, Gu MB. (2008) Single-stranded DNA aptamers specific for antibiotics tetracyclines. Bioorg Med Chem. 16: 7245-7253.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Niazi JH, Lee SJ, Gu MB. (2008) Single-stranded DNA aptamers specific for antibiotics tetracyclines. Bioorg Med Chem. 16: 7245-7253.
Kim YJ, Kim YS, Niazi JH, Gu MB. (2010) Electrochemical aptasensor for tetracycline detection. Bioprocess Biosyst Eng. 33:31-37.
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